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KMID : 0380620120440040452
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Korean Journal of Food Science and Technology
2012 Volume.44 No. 4 p.452 ~ p.459
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Resveratrol Induces Apoptosis through PI3K/Akt and p53 Signal Pathway in MDA-MB-231 Breast Cancer Cells
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Kwon Jung-Ki
Park Young-Suk
Park Byung-Kwon
Kim Byeong-Soo
Kim Sang-Ki
Jung Ji-Youn
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Abstract
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This study was conducted in order to investigate the effect of resveratrol on the induction of apoptosis in MDAMB- 231 breast cancer cells. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT) assay shows that cell viability significantly decreased in a dose and time-dependent manner. 4',6-diamidino-2-phenylindole (DAPI) staining shows significantly increased chromatin condensation in a dose and time-dependent manner. Resveratrol increased the expression of p53, cleaved-caspase-3, and cleaved-caspase-9, whereas the expression of PI3K/Akt decreased in a timedependent manner. We investigated the in vivo tumor growth inhibitory effect of resveratrol. Tumor volume was significantly decreased in the 50 mg/kg resveratrol-administration group compared to the control group. In the 50 mg/kg treated group. Apoptosis cells were frequently observed by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) assay. Immunohistochemistry staining shows increased the expression of p53, cytochrome-C, and cleaved-caspase-3 in the 50 mg/kg treated group. These results indicate that resveratrol induced apoptosis through PI3K/Akt and p53 signal pathway in MDA-MB-231 cell.
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KEYWORD
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resveratrol, apoptosis, PI3K/Akt, breast cancer cell, p53
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